In the U.S., the FDA has proposed to also accept the CODEX definition and the accompanying analytical method AOAC 2009.01 (and to reject an analytically unmanageable definition given by the Institute of Medicine in 2001). The proposed FDA rule change to 21 CFR part 101 will come into effect in 2016.
The U.K. has traditionally been the only country to use a completely different definition that is based on the use of the non-starch polysaccharide method to measure “fiber.” It is unclear at this time whether they will take this opportunity to harmonize with the rest of the world in this regard.
Megazyme is currently the only commercial supplier of the reagents required to run AOAC 2009.01. These are sold in the form of a kit (Integrated Total DF Assay Kit) that also contains a data booklet outlining the principle of the method and instructions for how to practically perform the procedure in the laboratory.
Dr. Mangan is currently employed as the R&D manager with Megazyme under Professor Barry McCleary, who developed the Integrated Total Dietary Fiber method (McCleary Method, AOAC 2009.01), along with CODEX approved methods for the measurement of resistant starch (AOAC 2002.02), β-glucan (AOAC 995.16), and fructo-oligosaccahrides (AOAC 999.03). Reach Dr. Mangan at [email protected].