Test samples were negative for indigenous Salmonella and had an average APC value of 2.7 logCFU/gram. For the 20 samples inoculated, Salmonella was recovered at 80 to 490 CFU/ml by direct plating. All direct unenriched samples were positive by PCR following the iQ-Check Salmonella II standard real-time PCR method and by the USDA-FSIS reference methods after standard enrichment procedure.
Collectively, these data illustrate that the iQ-Check Salmonella II standard real-time PCR method with modification can be used directly and reliably to detect Salmonella at a threshold of 100 CFU/ml (2.00 log CFU/ml) in poultry rinsates without enrichment.
Warren has provided technical support and guidance to the food and infection control industries for over 15 years and is currently vice president of government and regulatory affairs at Vanguard Sciences (formerly AEGIS Food Testing Laboratories). Reach her at firstname.lastname@example.org.
References Furnished Upon Request