In general, testing issues are mainly represented by the strongly varying matrices that have to be tested (matrix effect). The detection limit of any test, therefore, will vary depending on the sample matrix to be analyzed. Although providers of test kits often have a validation protocol to be submitted with the test kit upon request, laboratories using commercial test kits must establish in-house controls on detection limits and quantification limits in actual matrices. As there are no reference methods and no certified reference materials for allergens available, one has to keep in mind that results are not necessarily comparable between different test systems. Accredited methods should be used in official controls. The National Food Agency is accredited for the analysis of shellfish protein in foodstuffs.
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