Pathogenic Survivors

This study investigated the survival of Listeria monocytogenes at freezer temperatures (-20ºC) on the surface of ready-to-eat (RTE) meat products. An 18-hour culture of the pathogen was decimally diluted and inoculated (5.8 Log CFU/g) onto 45g cubes of RTE roast beef, hot dog, sausage, chicken breast and turkey.

The samples (vacuumed or non-vacuumed packaged) were stored at -20ºC and bacterial counts determined at day zero, seven, 14, 21, 28, 30, 60 and 90 by spread-plating onto modified Oxford media and then incubating plates at 37ºC for 48 hours and determining CFU/g.

At day seven, the results showed no significant difference in Lm counts between the different RTE meats tested. However, by day 28, Lm counts were significantly higher for the vacuum and non-vacuum RTE roast beef samples (6.7 Log CFU/g) from other meat samples (6.3 Log CFU/g). Furthermore, at day 28 all the RTE meat samples, except the vacuum-packaged turkey, had Lm counts greater than 0.5 Log CFU/g compared to day 0. Conversely, by day 90, the Lm counts had reached or were lower than the initial day 0 bacterial levels for all RTE meats. Throughout the study the vacuumed packaged roast beef, chicken and hot dogs Lm counts were significantly higher then the non-vacuumed packaged whereas the non-vacuumed packaged turkey Lm counts were significantly higher then the vacuumed packaged. Our study has shown that the ability of Lm to survive at freezer temperatures on RTE meats was dependent on the meat product and packaging condition.

Listeria monocytogenes, a gram-positive rod, has the capability to grow at refrigerator temperatures. Foods that are contaminated and consumed with Listeria have the ability to cause flu-like symptoms in healthy individuals. The disease, listeriosis, can cause more severe health effects for the elderly and immunocompromised persons. In the case of pregnant women, they can suffer from miscarriages or stillborn births.

Listeria monocytogenes has been associated with food recalls for a number of different products. However, in late 1998 to early 1999, there was a multistate outbreak of listeriosis identified in 22 states. The outbreak was traced to tainted hot dogs and deli meats. Two major recalls involved 30 million pounds each. According to the Centers for Disease Control and Prevention, tainted meat traced to a Michigan plant was responsible for 16 deaths, 6 miscarriages and 100 illnesses. Another outbreak in 10 states in 2000 resulted in 29 cases of listeriosis with 4 deaths and 3 miscarriages /stillbirths (Anonymous, 2000). As a result of reoccurring Listeria monocytogenes outbreaks, the USDA’s Food Safety and Inspection Service (FSIS) addressed the problem in a directive published in the Federal Register in 2001 and amended in 2003. It requires new performance standards for producing ready-to-eat meat products.

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